RNA Interference

The small size of MiniVectors allows them to achieve RNA interference in a robust and persistent platform.


Although their small size allows them to more easily enter a cell, siRNAs are highly unstable and rapidly degraded in vivo. In addition, after entering the cell the constructs are one-time-use and must be continually replenished to achieve sustained knockdown.

Expression of shRNA or miRNA from a MiniVector allows silencing to continue for longer time periods since multiple copies are continually expressed from each MiniVector. In addition, since the MiniVectors lack extraneous bacterial sequences, there is a reduced risk of silencing within the cell. Finally, the small, supercoiled MiniVector format is considerably more stable, easier to handle, and  resistant to serum degradation much longer than siRNA.

manufacturing-process

Benefits of the MiniVector System for RNA Interference Applications:


  • Unprecedented small size
    • MiniVectors as small as 300 bp can be generated
    • Physiological supercoiling makes them even more compact
  • Exceptional stability
    • The small size, circularity and supercoiling of MiniVectors confers unprecedented resistance to the shear force encountered during delivery
    • MiniVectors can resist degradation from serum exposure longer than siRNA
  • Enhanced transfection efficiency
    • The small and compact structure allows efficient entry into cells
    • Minimized bacterial sequences reduce silencing of the construct and potential immunogenicity