New MiniVector Product Literature Available

minivectors

A New Platform for:

  • RNA Interference
  • Gene Expression
  • Cell Labeling and Tracking
  • DNA Molecular Interactions

MiniVectors™ are small, circular, supercoiled DNA constructs ideal for shRNA knockdown, long term gene expression, cell labeling, and molecular studies of DNA structure and binding. In contrast to plasmids, MiniVectors are stripped down to the very essence of what you hope to deliver: the promoter and gene of interest.

Experiments have demonstrated that MiniVectors are a safe and effective platform for getting DNA into “difficult-to-transfect” cells, such as primary and stem cells.
MiniVectors transfect a wide range of cells types, have superior transfection efficiency, persist in cells, and can be used for targeted integration with CRISPR/TAL proteins, transposons, or zinc fingers.

These tiny, twisted up circles allow the first explorations of active DNA, and proteins or drugs that act on DNA.

Experience the Benefits of MiniVector Technology

  • Small! (as small as 250 bp)
  • Superior transfection efficiency compared to plasmids
  • Safer – No bacterial DNA or antibiotic resistance genes
  • Stealth backbone free of CpG motifs for reduced toxicity
  • Excellent biological and physical stability
  • No need for viral delivery vectors

MiniVectors™ are derived from a larger parent plasmid containing the origin and resistance genes necessary for amplification in bacteria. During production, a recombination event removes all of these undesirable components to leave only the pure, minimized vector containing your sequence of interest.

  • Irobalieva RN, Fogg JM, Catanese DJ, Sutthibutpong T, Chen M, Barker AK, Ludtke SJ, Harris SA, Schmid MF, Chiu W, Zechiedrich L. (2015) Structural Diversity of supercoiled DNA. Nature Comm [Epub ahead of print]. [article]
  • Stenler S, Blomberg P, Smith CI. (2014) Safety and efficacy of DNA vaccines: plasmids vs. minicircles. Hum Vaccin Immunother 10(5):1306-8. doi: 10.4161/hv.28077. [article]
  • Catanese DJ Jr, Fogg JM, Schrock DE 2nd, Gilbert BE, Zechiedrich L. (2012) Supercoiled Minivector DNA resists shear forces associated with gene therapy delivery. Gene Ther 19(1):94-100. doi: 10.1038/gt.2011.77. [article]
  • Zhao N, Fogg JM, Zechiedrich L, Zu Y. (2011) Transfection of shRNA-encoding Minivector DNA of a few hundred base pairs to regulate gene expression in lymphoma cells. Gene Ther 18(3):220-4. doi: 10.1038/gt.2010.123. [article]