Delivery of an expression cassette to a cell has traditionally been attempted in one of two basic formats. In one approach, large DNA constructs such as plasmids are combined with some form of transfection agent to compact the structure, neutralize charge, and allow the molecule to enter the cell. The alternative approach utilizes large, immunogenic viral particles to package and deliver the payload.
In contrast, MiniVectors™ are stripped down to the very essence of what you hope to deliver: the promoter and gene of interest. This results in minimal transfection agent requirements, exceptionally compact size, and elimination of bacterial or unnecessary sequences. In addition, MiniVectors have been shown to transfect even the most difficult cells lines.
TurboGFP Expression in Zebrafish Embryos Following MiniVector Injection
Benefits of the MiniVector System for Gene Delivery Applications:
- Unprecedented small size
- MiniVectors as small as 250 bp can be generated
- Physiological supercoiling makes them even more compact
- Exceptional stability
- The small size, circularity and supercoiling of MiniVectors confers unprecedented resistance to the shear force encountered during delivery
- MiniVectors can resist degradation in serum longer than plasmid DNA
- Enhanced transfection efficiency
- The small and compact structure allows efficient entry into cells
- Minimized bacterial sequences reduce silencing of the construct and potential immunogenicity